Mitotic Exit

This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications responsible for mitotic exit regulation; and ways to promote mitosis arrest in disease-associated conditions such as cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.