Metabolic Profiling
English | 2025 | ISBN: 1071643339 | 292 Pages | PDF (True) | 13 MB
English | 2025 | ISBN: 1071643339 | 292 Pages | PDF (True) | 13 MB
This second edition volume expands on the previous edition with new chapters and updated discussions on the latest advancements in the development and practice of metabolic phenotyping. The chapters in this book cover topics such as quality control in untargeted metabolic phenotyping; implementation of quality assurances processes; careful bio- and chemoinformatic data analysis; using reversed-phase and ion-pair LC-MS; quantitative lipid analysis using supercritical fluid chromatography (SFC-MS) and the targeted determination of metanephrines in urine via LC-MS/MS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.